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Feedback regulation of Caulobacter crescentus holdfast synthesis by flagellum assembly via the holdfast inhibitor HfiA.
- Source :
-
Molecular microbiology [Mol Microbiol] 2018 Oct; Vol. 110 (2), pp. 219-238. Date of Electronic Publication: 2018 Oct 05. - Publication Year :
- 2018
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Abstract
- To permanently attach to surfaces, Caulobacter crescentusproduces a strong adhesive, the holdfast. The timing of holdfast synthesis is developmentally regulated by cell cycle cues. When C. crescentusis grown in a complex medium, holdfast synthesis can also be stimulated by surface sensing, in which swarmer cells rapidly synthesize holdfast in direct response to surface contact. In contrast to growth in complex medium, here we show that when cells are grown in a defined medium, surface contact does not trigger holdfast synthesis. Moreover, we show that in a defined medium, flagellum synthesis and regulation of holdfast production are linked. In these conditions, mutants lacking a flagellum attach to surfaces over time more efficiently than either wild-type strains or strains harboring a paralyzed flagellum. Enhanced adhesion in mutants lacking flagellar components is due to premature holdfast synthesis during the cell cycle and is regulated by the holdfast synthesis inhibitor HfiA. hfiA transcription is reduced in flagellar mutants and this reduction is modulated by the diguanylate cyclase developmental regulator PleD. We also show that, in contrast to previous predictions, flagella are not necessarily required for C. crescentus surface sensing in the absence of flow, and that arrest of flagellar rotation does not stimulate holdfast synthesis. Rather, our data support a model in which flagellum assembly feeds back to control holdfast synthesis via HfiA expression in a c-di-GMP-dependent manner under defined nutrient conditions.<br /> (© 2018 John Wiley & Sons Ltd.)
- Subjects :
- Bacterial Adhesion drug effects
Bacterial Proteins genetics
Bacterial Proteins metabolism
Biofilms growth & development
Caulobacter crescentus genetics
Caulobacter crescentus growth & development
Cell Cycle drug effects
Culture Media pharmacology
Cyclic GMP analogs & derivatives
Cyclic GMP pharmacology
Feedback, Physiological
Mutation
Statistics, Nonparametric
Biofilms drug effects
Caulobacter crescentus drug effects
Flagella drug effects
Gene Expression Regulation, Bacterial drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2958
- Volume :
- 110
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 30079982
- Full Text :
- https://doi.org/10.1111/mmi.14099