Direct identification of Ruminococcus gnavus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on a positive anaerobic blood culture bottle.

Authors :: Fontanals D
Larruzea A
Sanfeliu I
Source :: Anaerobe [Anaerobe] 2018 Dec; Vol. 54, pp. 264-266. Date of Electronic Publication: 2018 Aug 01.
Publication Year :: 2018
Abstract: We report a case of bloodstream infection with the anaerobic bacterium Ruminococcus gnavus (R. gnavus), associated with intestinal perforation in a patient undergoing chemotherapy for multiple myeloma and cancer of the sigmoid colon. Gram staining of positive anaerobic blood cultures revealed both diplococci and short chains of gram-positive cocci. MALDI-TOF MS done directly on the blood culture bottle identified the bacterium as R. gnavus, and 16S rRNA gene sequencing confirmed the identification.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Subjects :: Aged
Anti-Bacterial Agents therapeutic use
Bacterial Typing Techniques methods
Gram-Positive Bacterial Infections diagnosis
Gram-Positive Bacterial Infections drug therapy
Humans
Male
Ruminococcus chemistry
Ruminococcus classification
Ruminococcus genetics
Blood Culture instrumentation
Gram-Positive Bacterial Infections microbiology
Ruminococcus isolation & purification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods

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