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Time Matters: Gene Editing at the Mouse 2-Cell Embryo Stage Boosts Knockin Efficiency.
- Source :
-
Cell stem cell [Cell Stem Cell] 2018 Aug 02; Vol. 23 (2), pp. 155-157. - Publication Year :
- 2018
-
Abstract
- Although endonuclease-mediated genome editing techniques offer significant improvement over traditional methods, they are still ineffective for introduction of large DNA sequences. Recently in Nature Biotechnology, Gu et al. (2018) developed a CRISPR-Cas strategy termed 2C-HR-CRISPR that generates fluorescent reporter tagging of genes with up to 95% knockin efficiency in mouse embryos.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Mice
Microinjections
CRISPR-Cas Systems
Gene Editing
Subjects
Details
- Language :
- English
- ISSN :
- 1875-9777
- Volume :
- 23
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cell stem cell
- Publication Type :
- Academic Journal
- Accession number :
- 30075125
- Full Text :
- https://doi.org/10.1016/j.stem.2018.07.008