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Protection of normal cells from irradiation bystander effects by silica-flufenamic acid nanoparticles.
- Source :
-
Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2018 Aug 03; Vol. 29 (8), pp. 130. Date of Electronic Publication: 2018 Aug 03. - Publication Year :
- 2018
-
Abstract
- The development of a myriad of nanoparticles types has opened new possibilities for the diagnostics and treatment of many diseases, especially for cancer. However, most of the researches done so far do not focus on the protection of normal cells surrounding a tumor from irradiation bystander effects that might lead to cancer recurrence. Gap-junctions are known to be involved in this process, which leads to genomic instability of neighboring normal cells, and flufenamic acid (FFA) is included in a new group of gap-junction blockers recently discovered. The present work explores the use of mesoporous silica nanoparticles MCM-41 functionalized with 3-Aminopropyltriethoxysilane (APTES) for anchoring the flufenamic acid for its prolonged and controlled release and protection from radiation bystander effects. MCM-41 and functionalized samples were structurally and chemically characterized with multiple techniques. The biocompatibility of all samples was tested in a live/dead assay performed in cultured MRC-5 and HeLa cells. HeLa cells cultured were exposed to 50 Gy of gamma-rays and the media transferred to fibroblast cells cultured separately. Our results show that MCM-41 and functionalized samples have high biocompatibility with MCR-5 and HeLa cells, and most importantly, the FFA delivered by these NPs was able to halt apoptosis, one of main bystander effects.
- Subjects :
- Bystander Effect drug effects
Cell Line
Fibroblasts drug effects
Fibroblasts radiation effects
Gamma Rays adverse effects
Humans
Materials Testing
Microscopy, Electron, Scanning
Bystander Effect radiation effects
Flufenamic Acid chemistry
Flufenamic Acid pharmacology
Nanoparticles chemistry
Silicon Dioxide chemistry
Silicon Dioxide pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4838
- Volume :
- 29
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of materials science. Materials in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 30074096
- Full Text :
- https://doi.org/10.1007/s10856-018-6134-5