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The distinct role and regulatory mechanism of IL-17 and IFN-γ in the initiation and development of plaque vs guttate psoriasis.
- Source :
-
Journal of dermatological science [J Dermatol Sci] 2018 Oct; Vol. 92 (1), pp. 106-113. Date of Electronic Publication: 2018 Jul 07. - Publication Year :
- 2018
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Abstract
- Background: Few studies have explored the differences of immunopathogenesis in plaque vs guttate psoriasis, especially on the inhibitory role of regulatory T cells (Tregs) on IL-17/ IFN-γ production and the impact of CD4+T cells on keratinocytes.<br />Objective: To investigate the percentage and inhibitory function of CD4+CD25 <superscript>high</superscript> Treg and differential expressions of IL-17/ IFN-γ in plaque vs guttate psoriasis, and the effects of CD4+T cells on the proliferation of keratinocytes.<br />Methods: Peripheral blood mononuclear cells (PBMCs) were prepared from patients with the plaque and guttate psoriasis. The percentage of CD4+CD25 <superscript>high</superscript> Tregs, IL-17/IFN-γ- producing CD4+ or CD8+T cells, and apoptosis and cell cycle of Hacat cells were determined by flow cytometry. The level of IFN-γ in supernatants was analyzed by ELISA.<br />Results: The percentage of CD4+CD25 <superscript>high</superscript> Tregs in plaque psoriasis was significantly increased, and they can inhibit IFN-γ production from CD4+CD25- effector T cells. The percentage of CD8+IFN-γ+cells was also significantly increased in plaque psoriasis, and these cells positively correlated with disease severity. The percentage of CD4+CD25 <superscript>high</superscript> Tregs was decreased and CD4+IFN-γ+/IFN-γ+IL-17+ cells were predominantly increased in guttate psoriasis. CD4+T cells from guttate psoriasis induce apoptosis of keratinocytes while they promote the proliferation of keratinocytes in plaque psoriasis by decreasing late apoptosis and increasing the percentage of G1 phase.<br />Conclusion: There was considerable discrepancy of the phenotype and function of T cells between plaque vs guttate psoriasis. IFN-γ and IL-17 from CD4+T cells play a crucial role in guttate psoriasis, however, IFN-γ and IL-17 from CD8+T cells are more important in the immunopathogenesis of plaque psoriasis.<br /> (Copyright © 2018. Published by Elsevier B.V.)
- Subjects :
- Adult
Apoptosis
CD8-Positive T-Lymphocytes immunology
Case-Control Studies
Cell Communication
Cell Line
Female
G1 Phase Cell Cycle Checkpoints
Humans
Interferon-gamma immunology
Interleukin-17 immunology
Keratinocytes immunology
Keratinocytes pathology
Male
Middle Aged
Phenotype
Psoriasis classification
Psoriasis immunology
Psoriasis pathology
Signal Transduction
Skin immunology
Skin pathology
T-Lymphocytes, Regulatory immunology
Young Adult
CD8-Positive T-Lymphocytes metabolism
Cell Proliferation
Interferon-gamma metabolism
Interleukin-17 metabolism
Keratinocytes metabolism
Psoriasis metabolism
Skin metabolism
T-Lymphocytes, Regulatory metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-569X
- Volume :
- 92
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of dermatological science
- Publication Type :
- Academic Journal
- Accession number :
- 30072243
- Full Text :
- https://doi.org/10.1016/j.jdermsci.2018.07.001