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Fast-Forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates.
- Source :
-
Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2019 Feb; Vol. 32 (2), pp. 142-156. Date of Electronic Publication: 2018 Dec 19. - Publication Year :
- 2019
-
Abstract
- Artificial small RNAs (sRNAs), including artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are used to silence viral RNAs and confer antiviral resistance in plants. Here, the combined use of recent high-throughput methods for generating artificial sRNA constructs and the Tomato spotted wilt virus (TSWV)-Nicotiana benthamiana pathosystem allowed for the simple and rapid identification of amiRNAs with high anti-TSWV activity. A comparative analysis between the most effective amiRNA construct and a syn-tasiRNA construct including the four most effective amiRNA sequences showed that both were highly effective against two different TSWV isolates. These results highlight the usefulness of this high-throughput methodology for the fast-forward identification of artificial sRNAs with high antiviral activity prior to time-consuming generation of stably transformed plants.
Details
- Language :
- English
- ISSN :
- 0894-0282
- Volume :
- 32
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular plant-microbe interactions : MPMI
- Publication Type :
- Academic Journal
- Accession number :
- 30070616
- Full Text :
- https://doi.org/10.1094/MPMI-05-18-0117-TA