Back to Search
Start Over
Expression of insulin-like growth factor I and its receptor in the liver of children with biopsy-proven NAFLD.
- Source :
-
PloS one [PLoS One] 2018 Jul 31; Vol. 13 (7), pp. e0201566. Date of Electronic Publication: 2018 Jul 31 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Background and Aims: Nonalcoholic fatty liver disease is one of the major complications of obesity, occurring already in pediatric age. Insulin like growth factor-I has been proposed as a potential therapeutic agent for its beneficial effect in experimental liver fibrosis. The aim of this work was to investigate the expression of insulin-like growth factor-I and its receptor in the liver of children with biopsy-proven nonalcoholic fatty liver disease and relate it to liver histological features.<br />Methods: 45 obese children and adolescents (14 females and 31 males) with nonalcoholic fatty liver disease were included. Insulin like growth factor-I and its receptor expression was evaluated in liver tissue by immunofluorescence and qPCR.<br />Results: The expression of insulin like growth factor-I and its receptor were significantly related to fibrosis and were higher in children with stage 3 fibrosis compared to stage 1 and 2 (p<0.001 and p = 0.007 respectively). mRNA of insulin like growth factor-I receptor was higher in more advanced stages of fibrosis (p<0.001). Furthermore, the expression of insulin like growth factor-I and its receptor in hepatic stellate cells, the cell type mostly involved in fibrosis progression, was significantly increased in stage 3 fibrosis compared to stage 1 (p = 0.01 and p = 0.008 respectively).<br />Conclusions: We demonstrated for the first time that insulin like growth factor-I and its receptor are upregulated in children with nonalcoholic fatty liver disease. These findings give a new hint for the potential therapeutic use of insulin like growth factor-I in pediatric nonalcoholic fatty liver disease complicated by liver fibrosis.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Adolescent
Biopsy
Child
Cohort Studies
Disease Progression
Female
Gene Expression
Humans
Insulin-Like Growth Factor I metabolism
Liver pathology
Liver Cirrhosis complications
Liver Cirrhosis genetics
Liver Cirrhosis metabolism
Liver Cirrhosis pathology
Male
Non-alcoholic Fatty Liver Disease complications
Non-alcoholic Fatty Liver Disease metabolism
Non-alcoholic Fatty Liver Disease pathology
Pediatric Obesity complications
Pediatric Obesity metabolism
Pediatric Obesity pathology
Receptor, IGF Type 1 metabolism
Insulin-Like Growth Factor I genetics
Liver metabolism
Non-alcoholic Fatty Liver Disease genetics
Pediatric Obesity genetics
Receptor, IGF Type 1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30063751
- Full Text :
- https://doi.org/10.1371/journal.pone.0201566