Back to Search
Start Over
The protective effects of sesamol in a neonatal rat model of necrotizing enterocolitis.
- Source :
-
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians [J Matern Fetal Neonatal Med] 2020 Mar; Vol. 33 (6), pp. 889-894. Date of Electronic Publication: 2018 Sep 27. - Publication Year :
- 2020
-
Abstract
- Background and aim: Necrotizing enterocolitis (NEC) is a severe gastrointestinal inflammatory disease associated with high rates of morbidity and mortality. Its pathophysiology includes hypoxic-ischemic injury that may be related to oxygen-derived free radical formation. Sesamol is considered to be an antioxidant and free radical scavenger with anti-inflammatory effects. The aim of this study is to investigate the effects of sesamol in a neonatal rat model of NEC. Materials and methods: The study included 1-day-old Wistar albino rat pups ( n = 34) that were randomly divided into four groups: Group 1 (NEC), group 2 (NEC + intraperitoneal sesamol), group 3 (NEC + oral sesamol), and a control group. NEC was induced by exposure to hypoxia/reoxygenation, following cold stress and hyperosmolar enteral formula feeding. Sesamol 100 mg kg <superscript>-1</superscript>  dose <superscript>-1</superscript> was administered intraperitoneally to group 2 and orally to group 3 for 3 days. On day 4 all rats were sacrificed. Histological injuries, the Bcl-2, caspase-3, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione-peroxidase (GSH-Px) activities were measured in intestinal samples. Results: The grade of intestinal damage, and Bcl-2 and caspase-3 levels in group 1 were significantly higher than in groups 2 and 3 and the control group, and intestinal damage was significantly more severe in group 1 than in groups 2 and 3. The MDA activity was significantly lower in groups 2 and 3 than in group 1 (112, 89, and 144 nmol mL <superscript>-1</superscript> , respectively). Groups 2 and 3 had significantly higher SOD and GSH-Px activities than group 1 (SOD: 1.75, 1.74, and 0.89 U mg <superscript>-1</superscript> ; GSH-Px: 114, 121, and 110 nmol of NADPH min <superscript>-1</superscript>  mg <superscript>-1</superscript> , respectively). Conclusions: The present findings highlight that sesamol has beneficial effects on intestinal injury in a rat model of NEC through its antioxidant and anti-inflammatory properties.
- Subjects :
- Administration, Oral
Animals
Animals, Newborn
Biomarkers metabolism
Enterocolitis, Necrotizing metabolism
Enterocolitis, Necrotizing pathology
Injections, Intraperitoneal
Intestinal Mucosa metabolism
Intestinal Mucosa pathology
Random Allocation
Rats
Rats, Wistar
Severity of Illness Index
Treatment Outcome
Anti-Inflammatory Agents therapeutic use
Antioxidants therapeutic use
Benzodioxoles therapeutic use
Enterocolitis, Necrotizing drug therapy
Phenols therapeutic use
Protective Agents therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4954
- Volume :
- 33
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
- Publication Type :
- Academic Journal
- Accession number :
- 30058400
- Full Text :
- https://doi.org/10.1080/14767058.2018.1506759