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High and efficient isomaltulose production using an engineered Yarrowia lipolytica strain.
- Source :
-
Bioresource technology [Bioresour Technol] 2018 Oct; Vol. 265, pp. 577-580. Date of Electronic Publication: 2018 Jun 25. - Publication Year :
- 2018
-
Abstract
- Isomaltulose is an ideal functional sweetener and has been approved as a safe sucrose substitute. It is produced mainly through sucrose isomerization catalyzed by sucrose isomerase. Here, to produce food-grade isomaltulose and improve its yield, the sucrose isomerase gene from Pantoea dispersa UQ68J was overexpressed in the non-pathogenic yeast Yarrowia lipolytica. When the engineered strain, S47, was fermented on 600 g/L sucrose in a 10-L bioreactor, a maximum isomaltulose concentration of 572.1 g/L was achieved. Sucrose isomerase activity was 7.43 U/mL, and yield reached 0.96 g/g. Moreover, monosaccharide byproducts were simultaneously transformed into intracellular lipids, thus reducing the production of undesirable compounds and resulting in high isomaltulose purity (97.8%) in the final broth. In summary, the bioprocess employed in this study provides an efficient alternative strategy for isomaltulose production.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Sucrose
Sweetening Agents
Isomaltose analogs & derivatives
Yarrowia
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 265
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 30056834
- Full Text :
- https://doi.org/10.1016/j.biortech.2018.06.081