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Therapeutic effects of vaccine derived from amastigote surface protein-2 (ASP-2) against Chagas disease in mouse liver.
- Source :
-
Cytokine [Cytokine] 2019 Jan; Vol. 113, pp. 285-290. Date of Electronic Publication: 2018 Jul 20. - Publication Year :
- 2019
-
Abstract
- This study investigated the efficacy of the vaccine in liver of mice infected with the Trypanosoma cruzi (T. cruzi) and immunized with AdASP-2. For this purpose, histopathological analysis and gene expression of COX-2, TNF-alpha, TNFR, iNOS, cytochrome C, caspase-3, TLR4, IL-6 and IL10 were evaluated. The following groups were used in this study: Group 1 - Control Group (CTRL) animals received AdβGal vehicle; Group 2 - Infected Group (TC) animals were infected with T. cruzi; Group 3 - Immunized Group (AdASP-2): animals were immunized by AdASP-2 vaccine; Group 4 - Immunized and Infected Group (AdASP-2+TC) animals were infected with T. cruzi and immunized by AdSP-2 vaccine. A significant decrease of amastigote nests was noticed in the group of animals that were immunized with AdASP-2 and infected on the same day. COX-2 and TNF-alpha gene expressions increased in TC group, whereas TNF-alpha decreased in the TC+AdASP-2 group. TNFR expression was high in AdASP-2+TC group. iNOS expression was high for all experimental groups whereas cytochrome C decreased for all experimental groups. Caspase 3 increased in TC and TC+AdASP-2 groups. The gene expression of TLR4 and IL-10 showed an increase in AdASP-2+TC group. Finally, hepatic fibrosis was noticed to TC and AdASP-2 + TC groups. Taken together, our results demonstrated that vaccination with AdASP-2 was effective against the acute phase of experimental Chagas disease as a result of a more powerful and rapid immune response closely related to expression of some inflammatory genes, such as iNOS, TNF-alpha, TLR 4, and IL-10.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adenoviridae
Animals
Caspase 3 immunology
Chagas Cardiomyopathy parasitology
Chagas Cardiomyopathy pathology
Chagas Cardiomyopathy prevention & control
Cyclooxygenase 2 immunology
Cytochromes c immunology
Cytokines immunology
Female
Liver parasitology
Liver pathology
Liver Cirrhosis parasitology
Liver Cirrhosis pathology
Liver Cirrhosis prevention & control
Mice
Nitric Oxide Synthase Type II immunology
Toll-Like Receptor 4 immunology
Chagas Cardiomyopathy immunology
Liver immunology
Liver Cirrhosis immunology
Neuraminidase immunology
Protozoan Vaccines immunology
Trypanosoma cruzi immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0023
- Volume :
- 113
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 30037707
- Full Text :
- https://doi.org/10.1016/j.cyto.2018.07.017