First detection of Pseudomonas aeruginosa ST2963 from hospital effluent: A draft genome analysis.

Authors :: Martins AF
de Lima Morales D
Mayer FQ
Fuentefria D
Corçao G
Source :: Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2018 Sep; Vol. 14, pp. 275-276. Date of Electronic Publication: 2018 Jul 20.
Publication Year :: 2018
Abstract: Objectives: Pseudomonas aeruginosa strains L25 and M12 were isolated from hospital effluent in southern Brazil.<br />Methods: The whole genomes of the isolates were sequenced using an Illumina MiSeq system. The data were analysed using SPAdes, Prokka and Geneious, and antimicrobial resistance genes were predicted using ResFinder. PubMLST protocols were used to define the sequence type (ST).<br />Results: Many multidrug efflux pump systems as well as various antimicrobial resistance genes were identified in the two P. aeruginosa strains. The strains were identified as ST2963, a novel carbapenem-resistant sequence type.<br />Conclusions: Here we describe the genome sequences of two carbapenem-resistant P. aeruginosa strains and characterised a novel sequence type (ST2963).<br /> (Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.)

Subjects :: Carbapenems pharmacology
Drug Resistance, Bacterial
Humans
Microbial Sensitivity Tests
Pseudomonas Infections microbiology
Pseudomonas aeruginosa isolation & purification
Genome, Bacterial
Pseudomonas aeruginosa genetics
Whole Genome Sequencing methods

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