Amperometric immunosensor for prolactin hormone measurement using antibodies loaded on a nano-Au monolayer modified ionic liquid carbon paste electrode.

Authors :: Beitollahi H
Nekooei S
Torkzadeh-Mahani M
Source :: Talanta [Talanta] 2018 Oct 01; Vol. 188, pp. 701-707. Date of Electronic Publication: 2018 Jun 15.
Publication Year :: 2018
Abstract: The fabrication of a novel electrochemical immunosensor for rapid and precise determination of prolactin was carried out using carbon paste electrode (CPE) consist of ionic liquid (IL) and graphite. Gold nanoparticles were employed as a modifier on the surface of CPE to immobilize the prolactin antibody (anti-PRL). The immunoassay was set up by sandwiching the antigen between prolactin antibody and the polyclonal anti-human-prolactin antibody labeled with horseradish peroxidase (HRP-labeled anti-PRL) as secondary antibody, on the surface of modified CPE. The reaction between O-aminophenol (OAP) and H <subscript>2</subscript> O <subscript>2</subscript> which is catalyzed by labeled HRP on the sandwich immunosensor generate a signal in differential pulse voltammetry (DPV) that is used to determine the concentration of prolactin. This immunosensor provides the measurement of prolactin concentration in a linear range of 25.0-2000.0 mIU L <superscript>-1</superscript> with a detection limit 12.5 mIU L <superscript>-1</superscript> . Moreover, it is applicable in the clinical assay of prolactin due to its high sensitivity and acceptable stability.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)

Subjects :: Aminophenols chemistry
Antibodies immunology
Armoracia enzymology
Biosensing Techniques methods
Electrodes
Horseradish Peroxidase chemistry
Hydrogen Peroxide chemistry
Immunoassay methods
Limit of Detection
Prolactin immunology
Electrochemical Techniques methods
Gold chemistry
Graphite chemistry
Ionic Liquids chemistry
Metal Nanoparticles chemistry
Prolactin analysis

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