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A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions.
- Source :
-
ELife [Elife] 2018 Jul 20; Vol. 7. Date of Electronic Publication: 2018 Jul 20. - Publication Year :
- 2018
-
Abstract
- Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLCĪµ that regulate the activation of PKD at trans-Golgi membranes. We further show that this molecular network coordinates the formation of TGN-derived Rab6-positive transport carriers delivering cargo for localized exocytosis at focal adhesions.<br />Competing Interests: SE, FC, GL, SS, MN, MS, MO, AH No competing interests declared<br /> (© 2018, Eisler et al.)
- Subjects :
- Cytoskeleton
HEK293 Cells
HeLa Cells
Humans
Phosphoinositide Phospholipase C genetics
Phosphoinositide Phospholipase C metabolism
Protein Transport
Rho Guanine Nucleotide Exchange Factors genetics
Rho Guanine Nucleotide Exchange Factors metabolism
TRPP Cation Channels genetics
TRPP Cation Channels metabolism
rab GTP-Binding Proteins genetics
rab GTP-Binding Proteins metabolism
rhoA GTP-Binding Protein genetics
rhoA GTP-Binding Protein metabolism
Focal Adhesions physiology
Microtubules metabolism
Protein Kinase C metabolism
Signal Transduction
trans-Golgi Network metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 30028295
- Full Text :
- https://doi.org/10.7554/eLife.35907