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Sodium Acetate, Sodium Acid Pyrophosphate, and Citric Acid Impacts on Isolated Peripheral Lymphocyte Viability, Proliferation, and DNA Damage.
- Source :
-
Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2018 Aug; Vol. 32 (8), pp. e22171. Date of Electronic Publication: 2018 Jul 18. - Publication Year :
- 2018
-
Abstract
- The present study examined the impacts of sodium acetate (SA), sodium acid pyrophosphate (SAPP), and citric acid (CA) on the viability, proliferation, and DNA damage of isolated lymphocytes in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were adopted to evaluate cell viability, while comet assay was employed to assess the genotoxic effects. The cells were incubated with different levels of SA (50, 100, and 200 mM), SAPP (25, 50, and 100 mM/L), or CA (100, 200, and 300 μg/mL). The lymphocytes treated with the tested food additives showed concentration-dependent decreases in both cell viability and proliferation. A concentration-dependent increase in LDH release was also observed. The comet assay results indicated that SA, SAPP, and CA increased DNA damage percentage, tail DNA percentage, tail length, and tail moment in a concentration-dependent manner. The current results showed that SA, SAPP, and CA are cytotoxic and genotoxic to isolated lymphocytes in vitro.<br /> (© 2018 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Cell Survival drug effects
Comet Assay
Dose-Response Relationship, Drug
Food Additives toxicity
L-Lactate Dehydrogenase metabolism
Limit of Detection
Lymphocytes cytology
Lymphocytes enzymology
Lymphocytes metabolism
Male
Rats, Sprague-Dawley
Cell Proliferation drug effects
Citric Acid toxicity
DNA Damage
Diphosphates toxicity
Lymphocytes drug effects
Sodium Acetate toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0461
- Volume :
- 32
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of biochemical and molecular toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 30019796
- Full Text :
- https://doi.org/10.1002/jbt.22171