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Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1).

Authors :
Chothe SK
Sebastian A
Thomas A
Nissly RH
Wolfgang D
Byukusenge M
Mor SK
Goyal SM
Albert I
Tewari D
Jayarao BM
Kuchipudi SV
Source :
Virology [Virology] 2018 Sep; Vol. 522, pp. 27-36. Date of Electronic Publication: 2018 Jul 06.
Publication Year :
2018

Abstract

Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1096-0341
Volume :
522
Database :
MEDLINE
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
30014855
Full Text :
https://doi.org/10.1016/j.virol.2018.06.015