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Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1).
- Source :
-
Virology [Virology] 2018 Sep; Vol. 522, pp. 27-36. Date of Electronic Publication: 2018 Jul 06. - Publication Year :
- 2018
-
Abstract
- Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cattle
Cluster Analysis
DNA, Viral genetics
Genotype
Genotyping Techniques methods
Herpesviridae Infections virology
Herpesvirus 1, Bovine isolation & purification
Minnesota
Pennsylvania
Polymerase Chain Reaction methods
Sequence Analysis, DNA
Whole Genome Sequencing
Cattle Diseases virology
Genetic Variation
Herpesviridae Infections veterinary
Herpesvirus 1, Bovine classification
Herpesvirus 1, Bovine genetics
Polymorphism, Single Nucleotide
Viral Vaccines genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 522
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 30014855
- Full Text :
- https://doi.org/10.1016/j.virol.2018.06.015