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Clinical validation of blood/brain glutamate grabbing in acute ischemic stroke.

Authors :: da Silva-Candal A
Pérez-Díaz A
Santamaría M
Correa-Paz C
Rodríguez-Yáñez M
Ardá A
Pérez-Mato M
Iglesias-Rey R
Brea J
Azuaje J
Sotelo E
Sobrino T
Loza MI
Castillo J
Campos F
Source :: Annals of neurology [Ann Neurol] 2018 Aug; Vol. 84 (2), pp. 260-273. Date of Electronic Publication: 2018 Aug 22.
Publication Year :: 2018
Abstract: Objective: Blood/brain-glutamate grabbing is an emerging concept in the treatment of acute ischemic stroke, where essentially the deleterious effects of glutamate after ischemia are ameliorated by coaxing glutamate to enter the bloodstream and thus reducing its concentration in the brain. Aiming to demonstrate the clinical efficacy of blood glutamate grabbers in patients with stroke, in this study, we resorted to a drug-repositioning strategy for the discovery of new glutamate-grabbing drugs.<br />Methods: The glutamate-grabbing ability of 1,120 compounds (90% of which were drugs approved by the US Food and Drug Administration) was evaluated during an in vitro high-throughput screening campaign. Subsequently, the protective efficacy of the selected drugs was probed in an ischemic animal model and finally tested in stroke patients.<br />Results: Riboflavin (vitamin B <subscript>2</subscript> ) was identified as the main hit compound. In ischemic animal models treated with riboflavin (1mg/kg), it was confirmed that blood glutamate reduction was associated with a significant reduction of infarct size. These results led to a randomized, double-blind, phase IIb clinical trial with patients with stroke. Fifty patients were randomized to 1 of the 2 study arms: the control group (placebo) and the experimental group (20mg of riboflavin [vitamin B <subscript>2</subscript> Streuli <superscript>@</superscript> ). Decrease in glutamate concentration was significantly greater (p < 0.029) in the treated group. Comparative analysis of the percentage improvement on the National Institutes of Health Stroke Scale score at discharge was slightly higher in the riboflavin-treated group than in the placebo group (33.7 ± 43.7 vs 48.9 ± 42.4%, p = 0.050).<br />Interpretation: This translational study represents the first human demonstration of the efficacy of blood glutamate grabbers in the treatment of patients with stroke, paving the way for the development of a promising novel protective therapy. Ann Neurol 2018;84:260-273.<br /> (© 2018 American Neurological Association.)