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A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques.
- Source :
-
Scientific reports [Sci Rep] 2018 Jul 16; Vol. 8 (1), pp. 10727. Date of Electronic Publication: 2018 Jul 16. - Publication Year :
- 2018
-
Abstract
- The recurrence of new human cases of Middle East respiratory syndrome coronavirus (MERS-CoV) underscores the need for effective therapeutic countermeasures. Nonhuman primate models are considered the gold standard for preclinical evaluation of therapeutic countermeasures. However, MERS-CoV-induced severe respiratory disease in humans is associated with high viral loads in the lower respiratory tract, which may be difficult to achieve in nonhuman primate models. Considering this limitation, we wanted to ascertain the effectiveness of using a MERS-CoV infectious clone (icMERS-0) previously shown to replicate to higher titers than the wild-type EMC 2012 strain. We observed respiratory disease resulting from exposure to the icMERS-0 strain as measured by CT in rhesus monkeys with concomitant detection of virus antigen by immunohistochemistry. Overall, respiratory disease was mild and transient, resolving by day 30 post-infection. Although pulmonary disease was mild, these results demonstrate for the first time the utility of CT imaging to measure disease elicited by a MERS-CoV infectious clone system in nonhuman primate models.
- Subjects :
- Animals
Coronavirus Infections pathology
Coronavirus Infections virology
Disease Models, Animal
Humans
Image Processing, Computer-Assisted
Lung pathology
Lung virology
Macaca mulatta
Middle East Respiratory Syndrome Coronavirus genetics
Middle East Respiratory Syndrome Coronavirus isolation & purification
Middle East Respiratory Syndrome Coronavirus physiology
RNA, Viral isolation & purification
Severity of Illness Index
Tomography, X-Ray Computed
Viral Load genetics
Virus Replication genetics
Coronavirus Infections diagnosis
Lung diagnostic imaging
Middle East Respiratory Syndrome Coronavirus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30013082
- Full Text :
- https://doi.org/10.1038/s41598-018-28900-1