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Sargassum serratifolium attenuates RANKL-induced osteoclast differentiation and oxidative stress through inhibition of NF-κB and activation of the Nrf2/HO-1 signaling pathway.
- Source :
-
Bioscience trends [Biosci Trends] 2018; Vol. 12 (3), pp. 257-265. - Publication Year :
- 2018
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Abstract
- Sargassum serratifolium C. Agardh is a marine brown alga that has long been used as an ingredient for food and medicine by many people living along Asian coastlines. Recently, various beneficial effects of extracts or compounds isolated from S. serratifolium have been reported, but their efficacies against bone destruction are unclear. Therefore, in this study, we investigated the inhibitory property of an ethanol extract of S. serratifolium (EESS) on osteoclast differentiation by focusing on the receptor activator of nuclear factor-κB ligand (RANKL)-stimulated osteoclastogenesis model using RAW 264.7 macrophages. Our results demonstrated that EESS reduced RANKL-induced osteoclast differentiation in RAW 264.7 cells, by inhibiting tartrate-resistant acid phosphatase (TRAP) activity and destroying the F-actin ring formation. EESS also attenuated RANKL-induced expressions of key osteoclast-specific genes, such as nuclear factor of activated T cells cytoplasmic 1 (NFATC1), TRAP, cathepsin K and matrix metalloproteinase-9. These effects were mediated by impaired nuclear translocation of nuclear factor (NF)-κB and suppression of IκB-α degradation. In addition, EESS effectively inhibited the production of reactive oxygen species (ROS) by RANKL, which was associated with enhanced expression of nuclear translocation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Overall, our findings provide evidence that EESS suppresses RANKL-induced osteoclastogenesis and oxidative stress through suppression of NF-κB and activation of Nrf2/HO-1 signaling pathway, indicating that S. serratifolium has a potential application the prevention and treatment of osteoclastogenic bone disease.
- Subjects :
- Actins metabolism
Animals
Cell Survival drug effects
Ethanol
Gene Expression Regulation drug effects
Mice
Osteoclasts drug effects
Osteoclasts metabolism
Osteogenesis drug effects
Osteogenesis genetics
Plant Extracts pharmacology
RAW 264.7 Cells
Reactive Oxygen Species metabolism
Signal Transduction
Cell Differentiation drug effects
Heme Oxygenase-1 metabolism
NF-E2-Related Factor 2 metabolism
NF-kappa B metabolism
Osteoclasts pathology
Oxidative Stress drug effects
RANK Ligand pharmacology
Sargassum chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1881-7823
- Volume :
- 12
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Bioscience trends
- Publication Type :
- Academic Journal
- Accession number :
- 30012915
- Full Text :
- https://doi.org/10.5582/bst.2018.01107