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Potential for Mitochondrial DNA Sequencing in the Differential Diagnosis of Gynaecological Malignancies.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2018 Jul 13; Vol. 19 (7). Date of Electronic Publication: 2018 Jul 13. - Publication Year :
- 2018
-
Abstract
- In the event of multiple synchronous gynecological lesions, a fundamental piece of information to determine patient management, prognosis, and therapeutic regimen choice is whether the simultaneous malignancies arise independently or as a result of metastatic dissemination. An example of synchronous primary tumors of the female genital tract most frequently described are ovarian and endometrial cancers. Surgical findings and histopathological examination aimed at resolving this conundrum may be aided by molecular analyses, although they are too often inconclusive. High mitochondrial DNA (mtDNA) variability and its propensity to accumulate mutations has been proposed by our group as a tool to define clonality. We showed mtDNA sequencing to be informative in synchronous primary ovarian and endometrial cancer, detecting tumor-specific mutations in both lesions, ruling out independence of the two neoplasms, and indicating clonality. Furthermore, we tested this method in another frequent simultaneously detected gynecological lesion type, borderline ovarian cancer and their peritoneal implants, which may be monoclonal extra-ovarian metastases or polyclonal independent masses. The purpose of this review is to provide an update on the potential use of mtDNA sequencing in distinguishing independent and metastatic lesions in gynecological cancers, and to compare the efficiency of molecular analyses currently in use with this novel method.
- Subjects :
- Diagnosis, Differential
Endometrial Neoplasms diagnosis
Endometrial Neoplasms genetics
Female
Genital Neoplasms, Female classification
Humans
Mutation
Ovarian Neoplasms diagnosis
Ovarian Neoplasms genetics
Sensitivity and Specificity
DNA, Mitochondrial genetics
Genital Neoplasms, Female diagnosis
Genital Neoplasms, Female genetics
Sequence Analysis, DNA methods
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 30011887
- Full Text :
- https://doi.org/10.3390/ijms19072048