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Estimate of the Prevalence of Anti-Gastric Parietal Cell Autoantibodies in Healthy Individuals Is Method Dependent.
- Source :
-
American journal of clinical pathology [Am J Clin Pathol] 2018 Aug 30; Vol. 150 (4), pp. 285-292. - Publication Year :
- 2018
-
Abstract
- Objectives: Anti-parietal cell antibodies (APCA) are a serologic marker of autoimmune gastritis. Their prevalence in healthy individuals is not well defined.<br />Methods: We evaluated APCA prevalence in 515 healthy blood-donors by rat/primate tissue indirect immunofluorescence (IIF), enzyme-linked immunosorbent assay (ELISA), and immunoblot.<br />Results: Fifty-three of 515 (10.3%) subjects were positive for APCA by at least one method: 18 only by ELISA, 10 by rodent tissue IIF, and one by primate tissue IIF; 18 were positive by ELISA and primate tissue IIF, and one by ELISA and rodent tissue IIF. Two were positive by both IIF methods, and three were triple positive. APCA positivity was confirmed by immunoblot in 100% of ELISA positive, in 95.8% of positive primate tissue IIF, and in 50% of positive rat tissue IIF.<br />Conclusions: A great discrepancy in APCA prevalence detected by different methods in this cohort was apparent. Thus, the results on APCA prevalence in healthy individuals are likely method-dependent.
- Subjects :
- Adult
Aged
Animals
Autoimmune Diseases blood
Autoimmune Diseases diagnosis
Autoimmune Diseases immunology
Biomarkers blood
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Gastritis blood
Gastritis diagnosis
Gastritis immunology
Healthy Volunteers
Humans
Immunoblotting
Male
Middle Aged
Primates
Rats
Autoantibodies blood
Parietal Cells, Gastric immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1943-7722
- Volume :
- 150
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- American journal of clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 30007281
- Full Text :
- https://doi.org/10.1093/ajcp/aqy061