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Possible mechanism of Vitis vinifera L. flavones on neurotransmitters, synaptic transmission and related learning and memory in Alzheimer model rats.
- Source :
-
Lipids in health and disease [Lipids Health Dis] 2018 Jul 04; Vol. 17 (1), pp. 152. Date of Electronic Publication: 2018 Jul 04. - Publication Year :
- 2018
-
Abstract
- Background: This study explored the possible mechanism of flavones from Vitis vinifera L. (VTF) on neurotransmitters, synaptic transmission and related learning and memory in rats with Alzheimer disease (AD).<br />Methods: The researchers injected amyloid-β <subscript>(25-35)</subscript> into the hippocampus to establish AD model rats. The Sprague-Dawley (SD) rats were divided into a control group, a donepezil group, an AD model group, a VTF low-dose group, a VTF medium-dose group and a VTF high-dose group. The researchers detected the activity of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) according to kit instructions. The protein expression of brain-derived neurotrophic factor (BDNF), synaptotagmin-1 (SYT1) and cyclic adenosine monophosphate response element binding protein (CREB) in the rats' hippocampi was detected by immunohistochemistry and Western blot, and the gene expression of cAMP-regulated enhancer (CRE) was detected by real-time quantitative polymerase chain reaction (PCR).<br />Results: VTF may enhance the protein expression of p-CREB, BDNF and SYT1 in rat hippocampi, depending on dose. The messenger RNA (mRNA) level of CREB was significantly higher in the VTF high-dose group than in the model group, which was consistent with the results of Western blotting. VTF may reduce the activity of AChE and increase that of ChAT in rat hippocampi. Finally, VTF effectively improved the learning and memory abilities of AD rats.<br />Conclusions: VTF can promote synaptic plasticity and indirectly affect the expression of cholinergic neurotransmitters, which may be one mechanism of VTF protection in AD rats.
- Subjects :
- Acetylcholine agonists
Acetylcholine biosynthesis
Acetylcholinesterase genetics
Acetylcholinesterase metabolism
Alzheimer Disease chemically induced
Alzheimer Disease genetics
Alzheimer Disease physiopathology
Amyloid beta-Peptides administration & dosage
Animals
Brain-Derived Neurotrophic Factor genetics
Brain-Derived Neurotrophic Factor metabolism
Choline O-Acetyltransferase genetics
Choline O-Acetyltransferase metabolism
Cyclic AMP Response Element Modulator genetics
Cyclic AMP Response Element Modulator metabolism
Cyclic AMP Response Element-Binding Protein genetics
Cyclic AMP Response Element-Binding Protein metabolism
Disease Models, Animal
Donepezil
Flavones isolation & purification
Gene Expression Regulation
Hippocampus drug effects
Hippocampus metabolism
Hippocampus physiopathology
Indans pharmacology
Male
Neurotransmitter Agents agonists
Neurotransmitter Agents biosynthesis
Nootropic Agents isolation & purification
Peptide Fragments administration & dosage
Piperidines pharmacology
Protein Aggregates
Rats
Rats, Sprague-Dawley
Synaptotagmin I genetics
Synaptotagmin I metabolism
Alzheimer Disease drug therapy
Flavones pharmacology
Maze Learning drug effects
Memory drug effects
Nootropic Agents pharmacology
Synaptic Transmission drug effects
Vitis chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1476-511X
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Lipids in health and disease
- Publication Type :
- Academic Journal
- Accession number :
- 29973282
- Full Text :
- https://doi.org/10.1186/s12944-018-0708-6