Nesterenkonia endophytica sp. nov., isolated from roots of Glycyrrhiza uralensis.

A Gram-positive and non-motile actinobacterium, designated strain EGI 60016 ^T , was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Xinyuan County, Xinjiang Province, China. The 16S rRNA gene sequence of strain EGI 60016 ^T was found to show 97.5 and 97.3 % sequence similarities to Nesterenkonia rhizosphaerae EGI 80099 ^T and Nesternkonia massiliensis NP1 ^T , respectively. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60016 ^T formed a distinct clade with N. rhizosphaerae EGI 80099 ^T and N. massiliensis NP1 ^T . The polar lipids detected for strain EGI 60016 ^T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, an unidentified lipid and an unidentified phospholipid. The DNA G+C content was determined to be 64.1 mol%. Other chemotaxonomic features of strain EGI 60016 ^T included MK-7, MK-8 and MK-9 as the respiratory quinones, and anteiso-C15 : 0 and anteiso-C17 : 0 as the major fatty acids. Based on the results of the phylogenetic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60016 ^T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia endophytica sp. nov. is proposed. The type strain is EGI 60016 ^T (=CCTCC AB 2017176 ^T =NBRC 112398 ^T ).