[Impact of Anthraquinone-2-sulfonic Acid on the MO Decolorization, Hydrogen Production and Energy Creation During Anaerobic Fermentation of Klebsiella oxytoca GS-4-08 with Sucrose].

The effect of 0-0.5 mmol·L ^-1 anthraquinone-2-sulfonic acid on anaerobic fermentation of Klebsiella oxytoca GS-4-08 was analyzed. By comparing bio-hydrogen production, carbon source degradation, and degradation products, the optimal concentration of 0.1 mmol·L ^-1 was acquired. Under this concentration, Klebsiella oxytoca GS-4-08 could decolorize 100% methyl orange within 10 h, degrade 92% sucrose within 25 h, meanwhile produce 0.117 mol·mol ^-1 ethanol, 0.116 mol·mol ^-1 acetic acid, 2.25 mol·mol ^-1 hydrogen. Besides, the promoting mechanism of AQS on MO decolorization and hydrogen production by Klebsiella oxytoca GS-4-08 was analyzed by comparing the experimental results and the literatures. The electron transfer and energy conversion are analyzed during the bacterial degradation process by converting the substances into COD; the optimal electron yield was 87.98% and the optimal energy generation rate was 802 kJ·mol ^-1 , and both were achieved under 0.1 mmol·L ^-1 of AQS. Using Klebsiella oxytoca GS-4-08, at least two types of biofuels could be produced via fermentation process, which showed prospects in future applications.