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In vitro and in vivo investigation of PLA/PCL scaffold coated with metformin-loaded gelatin nanocarriers in regeneration of critical-sized bone defects.
- Source :
-
Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2018 Oct; Vol. 14 (7), pp. 2061-2073. Date of Electronic Publication: 2018 Jun 28. - Publication Year :
- 2018
-
Abstract
- Large bone defects constitute a major challenge in bone tissue engineering and usually fail to heal due to the incomplete differentiation of recruited mesenchymal stem cells (MSCs) into osteogenic precursor cells. As previously proposed, metformin (MET) induces differentiation of MSCs into osteoblastic lineages in vitro. We fabricated a Poly (lactic acid) and Polycaprolactone (PLA/PCL) scaffold to deliver metformin loaded gelatin nanocarriers (MET/GNs) to critical-sized calvarial bone defects in a rat model. The scaffolds were evaluated regarding their morphology, porosity, contact angle, degradation rate, blood compatibility, biomechanical, cell viability and their osteogenic differentiation. In animal study, the defects were filled with autograft, scaffolds and a group was left empty. qRT-PCR analyses showed the expression level of osteogenic and angiogenic markers considerably increased in MET/GNs-PLA/PCL. The in vivo results showed that MET/GNs-PLA/PCL improved bone ingrowth, angiogenesis and defect reconstruction. Our results represent the applicability of MET/GNs-PLA/PCL for successful bone regeneration.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Bone Diseases pathology
Cell Differentiation
Coated Materials, Biocompatible chemistry
Hypoglycemic Agents pharmacology
In Vitro Techniques
Male
Materials Testing
Rats
Rats, Wistar
Tissue Engineering
Bone Diseases prevention & control
Bone Regeneration
Gelatin chemistry
Metformin pharmacology
Polyesters chemistry
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1549-9642
- Volume :
- 14
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 29964218
- Full Text :
- https://doi.org/10.1016/j.nano.2018.06.007