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Generation of the Adenovirus Vector-Mediated CRISPR/Cpf1 System and the Application for Primary Human Hepatocytes Prepared from Humanized Mice with Chimeric Liver.
- Source :
-
Biological & pharmaceutical bulletin [Biol Pharm Bull] 2018; Vol. 41 (7), pp. 1089-1095. - Publication Year :
- 2018
-
Abstract
- The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 system is now widely used as a genome editing tool. CRISPR-associated endonuclease in Prevotella and Francisella 1 (Cpf1) is a recently discovered Cas endonuclease that is designable and highly specific with efficiencies comparable to those of Cas9. Here we generated the adenovirus (Ad) vector carrying an Acidaminococcus sp. Cpf1 (AsCpf1) expression cassette (Ad-AsCpf1) for the first time. Ad-AsCpf1 was applied to primary human hepatocytes prepared from humanized mice with chimeric liver in combination with the Ad vector expressing the guide RNA (gRNA) directed to the Adeno-associated virus integration site 1 (AAVS1) region. The mutation rates were estimated by T7 endonuclease I assay around 12% of insertion/deletion (indel). Furthermore, the transduced human hepatocytes were viable (ca. 60%) at two weeks post transduction. These observations suggest that the Ad vector-mediated delivery of the CRISPR/AsCpf1 system provides a useful tool for genome manipulation of human hepatocytes.
- Subjects :
- Animals
Cell Line, Tumor
HEK293 Cells
Hepatocytes metabolism
Humans
Liver cytology
Mice
Primary Cell Culture
RNA, Guide, CRISPR-Cas Systems genetics
Transplantation Chimera
Adenoviridae genetics
Bacterial Proteins genetics
CRISPR-Cas Systems genetics
Endonucleases genetics
Genetic Vectors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1347-5215
- Volume :
- 41
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biological & pharmaceutical bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 29962404
- Full Text :
- https://doi.org/10.1248/bpb.b18-00222