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Recombinant club cell protein 16 (CC16) ameliorates cigarette smoke‑induced lung inflammation in a murine disease model of COPD.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2018 Aug; Vol. 18 (2), pp. 2198-2206. Date of Electronic Publication: 2018 Jun 25. - Publication Year :
- 2018
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Abstract
- Club cell protein (CC16) is expressed primarily by club cells possesses anti‑inflammatory properties and is located in the bronchiolar epithelium. Previous studies have demonstrated that CC16 deficiency is associated with the progression of chronic obstructive pulmonary disease (COPD). In the present study, the therapeutic effects of recombinant rat CC16 protein in mice with COPD were examined and the underlying mechanisms investigated. A total of 30 adult male C57/BL6 mice were randomly divided into three groups (10 mice/group). A mouse COPD model was generated by exposing 20 mice to cigarette smoke (CS) for 24 weeks. A total of 10 mice were treated intranasally with rCC16 (2.5 µg/g body weight) and control mice were exposed to normal room air. Results indicated that rCC16 treatment ameliorated pathological damage in the lungs and reduced the production of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑8, which were induced by CS exposure. After rCC16 administration, endogenous CC16 was upregulated and the body weight of COPD mice was increased, whereas the opposite was observed in CS‑exposed mice. Additionally, rCC16 treatment inhibited the DNA binding of NF‑κB/p65 in lung tissues and reduced nuclear translocation of NF‑κB/p65 in BALF and epithelial cells. Moreover, rCC16 treatment lead to a decrease in the total number of BALF cells, including macrophages, which was elevated in COPD mice. In conclusion, the present results demonstrate that rCC16 has therapeutic effects on COPD by downregulating pro‑inflammatory factors via the NF‑κB pathway.
- Subjects :
- Animals
Cigarette Smoking drug therapy
Cigarette Smoking pathology
Cytokines metabolism
Disease Models, Animal
Inflammation drug therapy
Inflammation metabolism
Inflammation pathology
Male
Mice
Pneumonia drug therapy
Pneumonia pathology
Pulmonary Disease, Chronic Obstructive drug therapy
Pulmonary Disease, Chronic Obstructive pathology
Recombinant Proteins pharmacology
Respiratory Mucosa pathology
Transcription Factor RelA metabolism
Cigarette Smoking metabolism
Pneumonia metabolism
Pulmonary Disease, Chronic Obstructive metabolism
Respiratory Mucosa metabolism
Signal Transduction drug effects
Uteroglobin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 29956762
- Full Text :
- https://doi.org/10.3892/mmr.2018.9216