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Alternative Method to Detect Neuronal Degeneration and Amyloid β Accumulation in Free-Floating Brain Sections With Fluoro-Jade.
- Source :
-
ASN neuro [ASN Neuro] 2018 Jan-Dec; Vol. 10, pp. 1759091418784357. - Publication Year :
- 2018
-
Abstract
- Fluoro-Jade is a fluorescein-derived fluorochrome which specifically binds to damaged neurons. Due to this characteristic, it is commonly used for the histochemical detection and quantification of neurodegeneration in mounted brain sections. Here, we describe an alternative and simpler histochemistry protocol based on the use of free-floating brain sections. For this purpose, we have used brain slices from wild-type and 5xFAD mice as well as from mice that received an intracerebral injection of oligomeric amyloid beta peptides. We observed that our histochemistry staining procedure allows for a well-defined labeling of degenerating neurons providing a better signal-to-noise ratio staining than the commonly used one. In addition, our modified protocol demonstrates the ability of Fluoro-Jade C to also fluorescently label amyloid beta plaques.
- Subjects :
- Amyloid beta-Peptides toxicity
Amyloid beta-Protein Precursor genetics
Animals
Brain drug effects
Disease Models, Animal
Gene Expression Regulation drug effects
Gene Expression Regulation genetics
Humans
In Vitro Techniques
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Mutation genetics
Nerve Degeneration genetics
Peptide Fragments toxicity
Presenilin-1 genetics
Amyloid beta-Peptides metabolism
Brain metabolism
Fluoresceins metabolism
Nerve Degeneration pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1759-0914
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- ASN neuro
- Publication Type :
- Academic Journal
- Accession number :
- 29950099
- Full Text :
- https://doi.org/10.1177/1759091418784357