Back to Search
Start Over
Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy.
- Source :
-
Nanomedicine (London, England) [Nanomedicine (Lond)] 2018 Jun; Vol. 13 (11), pp. 1333-1348. - Publication Year :
- 2018
-
Abstract
- Aim: To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI.<br />Materials & Methods: Astrocytes were exogenously labeled with MRI-compatible iron-oxide MNPs prior to intra-construct incorporation within a 3D collagen hydrogel.<br />Results: A connective, complex cellular network was clearly observable within the 3D constructs, with high cellular viability. MNP accumulation in astrocytes provided a hypointense MRI signal at 24 h & 14 days.<br />Conclusion: Our findings support the concept of developing a 3D construct possessing the dual advantages of (i) support of long-term cell survival of neural populations with (ii) the potential for noninvasive MRI-tracking of intra-construct cells for neuroregenerative applications.
- Subjects :
- Animals
Astrocytes chemistry
Astrocytes transplantation
Cell Proliferation drug effects
Cell Survival drug effects
Cell Tracking
Humans
Hydrogels chemistry
Hydrogels pharmacology
Magnetic Resonance Imaging
Magnetite Nanoparticles chemistry
Microscopy, Electron, Transmission
Nerve Degeneration drug therapy
Nerve Degeneration pathology
Neurons chemistry
Neurons transplantation
Polymers administration & dosage
Polymers chemistry
Rats
Astrocytes ultrastructure
Cell- and Tissue-Based Therapy
Magnetite Nanoparticles administration & dosage
Nerve Degeneration diagnostic imaging
Neurons ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1748-6963
- Volume :
- 13
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nanomedicine (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 29949467
- Full Text :
- https://doi.org/10.2217/nnm-2017-0347