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Enterohemorrhagic Escherichia coli O157 outer membrane vesicles induce interleukin 8 production in human intestinal epithelial cells by signaling via Toll-like receptors TLR4 and TLR5 and activation of the nuclear factor NF-κB.
- Source :
-
International journal of medical microbiology : IJMM [Int J Med Microbiol] 2018 Oct; Vol. 308 (7), pp. 882-889. Date of Electronic Publication: 2018 Jun 19. - Publication Year :
- 2018
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Abstract
- Proinflammatory cytokines play important roles in the pathogenesis of diseases caused by enterohemorrhagic Escherichia coli (EHEC) O157, but the spectrum of bacterial components involved in the proinflammatory responses is not fully understood. Here, we investigated the abilities of outer membrane vesicles (OMVs), nanoparticles released by EHEC O157 during growth, to induce production of proinflammatory cytokines in human intestinal epithelial cells. OMVs from both EHEC O157:H7 and sorbitol-fermenting (SF) EHEC O157:H <superscript>-</superscript> induced production of interleukin-8 (IL-8) in Caco-2, HCT-8, and HT-29 intestinal epithelial cell lines. H7 flagellin was the key IL-8-inducing component of EHEC O157:H7 OMVs, whereas cytolethal distending toxin V and O157 lipopolysaccharide (LPS) largely contributed to IL-8 production elicited by flagellin-lacking OMVs from SF EHEC O157:H <superscript>-</superscript> . The H7 flagellin-mediated signaling via Toll-like receptor (TLR) 5, and O157 LPS-mediated signaling via TLR4/MD-2 complex, which were followed by activation of the nuclear factor NF-κB were major pathways underlying IL-8 production induced by EHEC O157 OMVs. The proinflammatory and immunomodulatory capacities of EHEC O157 OMVs have pathogenetic implications and support the OMVs as suitable vaccine candidates.<br /> (Copyright © 2018 Elsevier GmbH. All rights reserved.)
- Subjects :
- Bacterial Outer Membrane Proteins metabolism
Caco-2 Cells
Cell Line, Tumor
Cell Membrane metabolism
Escherichia coli Infections pathology
Escherichia coli Proteins metabolism
Flagellin metabolism
HT29 Cells
Humans
Intestinal Mucosa cytology
Intestinal Mucosa microbiology
Signal Transduction
Virulence Factors metabolism
Epithelial Cells metabolism
Escherichia coli Infections microbiology
Escherichia coli O157 pathogenicity
Interleukin-8 biosynthesis
Intestinal Mucosa pathology
NF-kappa B metabolism
Toll-Like Receptor 4 metabolism
Toll-Like Receptor 5 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0607
- Volume :
- 308
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of medical microbiology : IJMM
- Publication Type :
- Academic Journal
- Accession number :
- 29934223
- Full Text :
- https://doi.org/10.1016/j.ijmm.2018.06.004