[Metabolomics analysis on serum biomarkers timing characteristics of type 2 diabetic rats intervened by exercise].

Objective: To analyze the combined features of serum biomarker of exercise-intervened type 2 diabetes mellitus (T2DM) rats at different time point.
Methods: Ninety SD rats randomly selected from 100 were fed with high glucose and fat diet for 4 weeks, and then received intraperitoneal injection of streptozotocin(STZ) according to 40 mg/kg by weight; after modeling for one week, Seventy-one rats were successfully modeled among which intervened with swimming at three different time points (2w, 4w and 8w). Eighty-one rats were finally divided into 8 groups as below:Normal control group (C ₀ , n =10);DM model comparison group (DC ₀ , n =10); DM model comparison 2w group (DC ₂ , n =11); DM model comparison 4w group (DC ₄ , n =10); DM model comparison 8w group (DC ₈ , n =9); DM exercise intervention 2w group (DE ₂ , n =11); DM exercise intervention 4w group (DE ₄ , n =10); DM exercise intervention 8w group (DE ₈ , n =11).The UPLC/Q-TOF MS technique was used to conduct metabonomics test of serum of rats to analyze the combined features of serum biomarkers under exercise intervention at different time points.
Results: According to fold change, we got the biomarker combinations (with 15 specific substances in each combination) of exercise intervention at three time points. In these serum biomarker combinations that were specific at different time points, a majority of metabolites appeared simultaneously at different time points but with obvious difference of fold change. Fold change of monoglyceride(24:6) and gluconic acid were most evident respectively at 2w and 4w intervention time points,Eicosapentaenoic Acid (EPA) andlinolenic acid(LA) contents might approach the normal level at 4~8 time point.
Conclusions: Combined features of serum biomarker of exercise-intervened T2DM rats are specific at different time point. Both EPA and LA are common substances significantly up-regulated while MonoglycerideMG(24:6), Gluconic acid, Propionyl-L-carnitine(PLC), Arginine(Arg) and Sphingosine-1-phosphate (SPP) are common substances significantly down-regulated at three time points.