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Fibrin Glue-aided, Instant Epicardial Placement Enhances the Efficacy of Mesenchymal Stromal Cell-Based Therapy for Heart Failure.
- Source :
-
Scientific reports [Sci Rep] 2018 Jun 21; Vol. 8 (1), pp. 9448. Date of Electronic Publication: 2018 Jun 21. - Publication Year :
- 2018
-
Abstract
- Transplantation of mesenchymal stromal cells (MSCs) is a promising new therapy for heart failure. However, the current cell delivery routes result in poor donor cell engraftment. We therefore explored the role of fibrin glue (FG)-aided, instant epicardial placement to enhance the efficacy of MSC-based therapy in a rat ischemic cardiomyopathy model. We identified a feasible and reproducible method to instantly produce a FG-MSC complex directly on the heart surface. This complex exhibited prompt, firm adhesion to the heart, markedly improving initial retention of donor MSCs compared to intramyocardial injection. In addition, maintenance of retained MSCs was enhanced using this method, together contributing the increased donor cell presence. Such increased donor cell quantity using the FG-aided technique led to further improved cardiac function in association with augmented histological myocardial repair, which correlated with upregulation of tissue repair-related genes. We identified that the epicardial layer was eliminated shortly after FG-aided epicardial placement of MSCs, facilitating permeation of the donor MSC's secretome into the myocardium enabling myocardial repair. These data indicate that FG-aided, on-site, instant epicardial placement enhances MSC engraftment, promoting the efficacy of MSC-based therapy for heart failure. Further development of this accessible, advanced MSC-therapy is justified.
- Subjects :
- Animals
Cell Adhesion
Cells, Cultured
Female
Fibrin Tissue Adhesive therapeutic use
Male
Mesenchymal Stem Cells drug effects
Mesenchymal Stem Cells physiology
Pericardium drug effects
Rats
Rats, Inbred Lew
Fibrin Tissue Adhesive pharmacology
Heart Failure therapy
Mesenchymal Stem Cell Transplantation methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29930312
- Full Text :
- https://doi.org/10.1038/s41598-018-27881-5