Structure of the O-specific polysaccharide from Azospirillum fermentarium CC-LY743 T .

O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of nitrogen-fixing bacterium Azospirillum fermentarium CC-LY743 ^T (IBPPM 578) and was studied by sugar analysis along with ¹ H and ¹³ C NMR spectroscopy, including ¹ H, ¹ H COSY, TOCSY, ROESY, and ¹ H, ¹³ C HSQC and HMBC experiments. The polysaccharide was found to be linear and to consist of alterating α-l-fucose and α-d-mannose residues in tetrasaccharide repeating units of the following structure: →2)-α-D-Manp-(1 → 3)-α-L-Fucp-(1 → 3)-α-D-Manp-(1 → 3)-α-L-Fucp-(1→.
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