A new xanthene-based two-photon fluorescent probe for the imaging of 1,4-dithiothreitol (DTT) in living cells.

Authors :: Wang C
Dong B
Kong X
Zhang N
Song W
Lin W
Source :: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2018 Sep; Vol. 33 (6), pp. 1048-1053. Date of Electronic Publication: 2018 Jun 21.
Publication Year :: 2018
Abstract: 1,4-Dithiothreitol (DTT) has wide applications in cell biology and biochemistry. Development of effective methods for monitoring DTT in biological systems is important for the safe handling and study of toxicity to humans. Herein, we describe a two-photon fluorescence probe (Rh-DTT) to detect DTT in living systems for the first time. Rh-DTT showed high selectivity and sensitivity to DTT. Rh-DTT can be successfully used for the two-photon imaging of DTT in living cells, and also can detect DTT in living tissues and mice.<br /> (© 2018 John Wiley & Sons, Ltd.)

Subjects :: Animals
Cell Survival
Female
Fluorescent Dyes chemical synthesis
Humans
Liver chemistry
Mice
Mice, Inbred BALB C
Molecular Structure
Tumor Cells, Cultured
Dithiothreitol analysis
Fluorescent Dyes chemistry
Photons
Xanthenes chemistry

Language :: English
ISSN :: 1522-7243
Volume :: 33
Issue :: 6
Database :: MEDLINE
Journal :: Luminescence : the journal of biological and chemical luminescence
Publication Type :: Academic Journal
Accession number :: 29927540
Full Text :: https://doi.org/10.1002/bio.3507

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