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[The effects of arecoline on the lipid metabolism of 3T3-L1 adipocytes].

Authors :
Ling HY
He J
Yang SS
Zhang KF
He JQ
Zhu ZM
Feng SD
Source :
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology [Zhongguo Ying Yong Sheng Li Xue Za Zhi] 2017 Jan 08; Vol. 33 (1), pp. 22-25.
Publication Year :
2017

Abstract

Objective: To observe the effects of arecoline on lipid metabolism in 3T3-L1 adipocytes and explore its possible mechanisms.<br />Methods: 3T3-L1 pre-adipocytes were induced into adipocytes with the classic "cocktail" method, subsequently, adipocytes were treated with arecoline at the concentrations of 0, 25, 50 and 100 μ mol/L for 72 hours. After 72 hours, cell vability was measured with MTT method, lipid droplet accumulation in the cytoplasm was observed with oil red O staining, the protein expression of fatty acid synthase (FAS), adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) were detected by Western blot assay.<br />Results: There were a large number of lipid droplets in the cytoplasm in the differentiated 3T3-L1 adipocytes. MTT results showed that 0~100 μ mol/L arecoline had no significant effect on cell vability; oil red O staining found arecoline reduced lipid amount in 3T3-L1 adipocytes; Western blot results showed that compared with 0 μ mol/L arecoline group (the control group), arecoline significantly reduced the protein level of FAS and increased the protein levels of ATGL and HSL, and 50 μ mol/L arecoline group was the most significant.<br />Conclusions: Arecoline significantly increased lipolysis of 3T3-L1 adipocyte, which might be associated with decreased the FAS expression of key enzyme of lipid synthesis and increased the ATGL and HSL expression of key enzyme of adipolysis.

Details

Language :
Chinese
ISSN :
1000-6834
Volume :
33
Issue :
1
Database :
MEDLINE
Journal :
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Publication Type :
Academic Journal
Accession number :
29926602
Full Text :
https://doi.org/10.12047/j.cjap.5448.2017.005