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Virus RNA Load in Patients with Tick-Borne Encephalitis, Slovenia.
- Source :
-
Emerging infectious diseases [Emerg Infect Dis] 2018 Jul; Vol. 24 (7), pp. 1315-1323. - Publication Year :
- 2018
-
Abstract
- We determined levels of tick-borne encephalitis (TBE) virus (TBEV) RNA in serum samples obtained from 80 patients during the initial phase of TBE in Slovenia. For most samples, levels were within the range of 3-6 log <subscript>10</subscript> copies RNA/mL. Levels were higher in female patients than in male patients, but we found no association between virus load and several laboratory and clinical parameters, including severity of TBE. However, a weak humoral immune response was associated with a more severe disease course, suggesting that inefficient clearance of virus results in a more serious illness. To determine whether a certain genetic lineage of TBEV had a higher virulence potential, we obtained 56 partial envelope protein gene sequences by directly sequencing reverse transcription PCR products from clinical samples of patients. This method provided a large set of patient-derived TBEV sequences. We observed no association between phylogenetic clades and virus load or disease severity.
- Subjects :
- Adult
Antibodies, Viral immunology
Female
Humans
Immunoglobulin G immunology
Male
Middle Aged
Phylogeny
Slovenia epidemiology
Encephalitis Viruses, Tick-Borne genetics
Encephalitis Viruses, Tick-Borne immunology
Encephalitis, Tick-Borne epidemiology
Encephalitis, Tick-Borne virology
RNA, Viral
Viral Load
Subjects
Details
- Language :
- English
- ISSN :
- 1080-6059
- Volume :
- 24
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Emerging infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 29912706
- Full Text :
- https://doi.org/10.3201/eid2407.180059