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MiR-378 and BMP-Smad can influence the proliferation of sheep myoblast.
- Source :
-
Gene [Gene] 2018 Oct 20; Vol. 674, pp. 143-150. Date of Electronic Publication: 2018 Jun 14. - Publication Year :
- 2018
-
Abstract
- MicroRNA (miRNA) is a sort of endogenous ~20-25 nt non-coding RNAs, and it can regulate a variety of biological events. We found the miR-378 may involve in regulating the muscle development of sheep during our previous research. However, the molecular mechanism of miR-378 regulating myoblast proliferation is still unclear. In this research, we predicted that BMP2 (Bone morphogenetic protein 2) was the target gene of miR-378 and the BMP-Smad signal pathway that BMP2 participated in playing an important role in the muscle development. Therefore, we tried to determine whether miR-378 influence myoblast proliferation of sheep through the BMP-Smad signal pathway. The results indicated that inhibit BMP-Smad signal pathway by interfering Smad4 to promote proliferation of sheep myoblasts; promote BMP-Smad signal pathway by interfering Smad7 to inhibit proliferation of sheep myoblasts; over-expression miR-378 promotes BMP-Smad signal pathway and myoblast proliferation in sheep; interfering miR-378 inhibits BMP-Smad signal pathway and myoblast proliferation in sheep. However, when both of which functioned at the myoblast, miR-378 could not fully depend on BMP-Smad signal pathway to regulate myoblast proliferation. In sum, both miR-378 and BMP-Smad can influence the proliferation of myoblast, but miR-378 does not target the 3' UTR of sheep BMP2.<br /> (Copyright © 2018. Published by Elsevier B.V.)
- Subjects :
- 3' Untranslated Regions
Animals
Bone Morphogenetic Protein 2 physiology
Cell Proliferation
Cells, Cultured
Myoblasts, Skeletal cytology
Sheep
Signal Transduction
Smad4 Protein antagonists & inhibitors
Smad4 Protein genetics
Smad7 Protein antagonists & inhibitors
Smad7 Protein genetics
Bone Morphogenetic Protein 2 genetics
MicroRNAs metabolism
Myoblasts, Skeletal metabolism
Smad4 Protein metabolism
Smad7 Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0038
- Volume :
- 674
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 29908283
- Full Text :
- https://doi.org/10.1016/j.gene.2018.06.039