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Splitting of circulating red blood cells as an in vivo mechanism of erythrocyte maturation in developing zebrafish, chick and mouse embryos.
- Source :
-
The Journal of experimental biology [J Exp Biol] 2018 Aug 10; Vol. 221 (Pt 15). Date of Electronic Publication: 2018 Aug 10. - Publication Year :
- 2018
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Abstract
- Nucleated circulating red blood cells (RBCs) of developing zebrafish, chick and mouse embryos can actively proliferate. While marrow- or organ-mediated erythropoiesis has been widely studied, transforming in vivo processes of circulating RBCs are under little scrutiny. We employed confocal, stereo- and electron microscopy to document the maturation of intravascular RBCs . In zebrafish embryos (32-72 h post-fertilization), RBC splitting in the caudal vein plexus follows a four-step program: (i) nuclear division with continued cytoplasmic connection between somata; (ii) dumbbell-shaped RBCs tangle at transluminal vascular pillars; (iii) elongation; and (iv) disruption of soma-to-soma connection. Dividing RBCs of chick embryos, however, retain the nucleus in one of their somata. Here, RBC splitting acts to pinch off portions of cytoplasm, organelles and ribosomes. Dumbbell-shaped primitive RBCs re-appeared as circulation constituents in mouse embryos. The splitting of circulating RBCs thus represents a biologically relevant mechanism of RBC division and maturation during early vertebrate ontogeny.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2018. Published by The Company of Biologists Ltd.)
Details
- Language :
- English
- ISSN :
- 1477-9145
- Volume :
- 221
- Issue :
- Pt 15
- Database :
- MEDLINE
- Journal :
- The Journal of experimental biology
- Publication Type :
- Academic Journal
- Accession number :
- 29903841
- Full Text :
- https://doi.org/10.1242/jeb.184564