[Improving the expression of TP53INP1 in A549 cells enhances the sensitivity of cells to arsenic treatment].

Objective: To explore if improving the expression of TP53INP1 could enhance the sensitivity of A549 cells to arsenic.
Methods: The eukaryotic express vector containing TP53INP1 gene was transferred into A549 cells by using lentivirus vector. Cell apoptosis and cell viability after arsenic treatment were assessed by flow cytometry and MTT assay, respectively.
Results: The protein expression level of TP53INP1 was increased in A549 cells transferred with eukaryotic express vector containing TP53INP1 gene, which led to an increase in apoptosis and a decrease in cell viability. Compared with A549 cells, significant increase in apoptosis was found in A549-TP53INP1 cells when treated with As&#95;2O&#95;3( 5- 40 μmol/L). In addiation, the IC50 of As&#95;2O&#95;3 in A549-TP53INP1(( 44. 64 ± 6. 84) μmol/L) cells was significantly lower than that of the A549 group(( 54. 25 ± 6. 13) μmol/L)( P < 0. 05).
Conclusion: Enhancement of TP53INP1 can significantly improve apoptosis response and enhance sensitivity of A549 cells to arsenic. It is suggested that TP53INP1 could be used as a new target in arsenic-based cancer treatment.