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Protective effect of crocin on ultraviolet B‑induced dermal fibroblast photoaging.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2018 Aug; Vol. 18 (2), pp. 1439-1446. Date of Electronic Publication: 2018 Jun 11. - Publication Year :
- 2018
-
Abstract
- Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS), resulting in the aging of dermal fibroblasts. Crocin, a bioactive constituent of Crocus sativus, possesses anti‑oxidation effects. The purpose of the present study was to evaluate the protective effect of crocin on UVB‑induced dermal fibroblast photoaging. Human dermal fibroblasts were isolated and cultured with different concentrations of crocin prior to and following exposure to UVB irradiation. The senescent phenotypes of cells were evaluated, including cell proliferation, cell cycle, senescence‑associated β‑galactosidase (SA‑β‑gal) expression, intracellular ROS, expression of antioxidant protein glutathione peroxidase 1 (GPX‑1) and extracellular matrix protein collagen type 1 (Col‑1). Crocin rescued the cell proliferation inhibited by UVB irradiation, prevented cell cycle arrest and markedly decreased the number of SA‑β‑gal‑positive cells. In addition, crocin reduced UVB‑induced ROS by increasing GPX‑1 expression and other direct neutralization effects. Furthermore, crocin promoted the expression of the extracellular matrix protein Col‑1. Crocin could effectively prevent UVB‑induced cell damage via the reduction of intracellular ROS; thus, it could potentially be used in the prevention of skin photoaging.
- Subjects :
- Cell Cycle drug effects
Cell Cycle genetics
Cell Proliferation drug effects
Collagen Type I agonists
Collagen Type I genetics
Collagen Type I metabolism
Crocus chemistry
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Fibroblasts cytology
Fibroblasts metabolism
Fibroblasts radiation effects
Foreskin cytology
Gene Expression Regulation
Glutathione Peroxidase genetics
Glutathione Peroxidase metabolism
Humans
Male
Primary Cell Culture
Reactive Oxygen Species agonists
Reactive Oxygen Species metabolism
Signal Transduction
Skin Aging drug effects
Ultraviolet Rays adverse effects
beta-Galactosidase antagonists & inhibitors
beta-Galactosidase genetics
beta-Galactosidase metabolism
Glutathione Peroxidase GPX1
Carotenoids pharmacology
Cellular Senescence drug effects
Fibroblasts drug effects
Radiation-Protective Agents pharmacology
Reactive Oxygen Species antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 29901204
- Full Text :
- https://doi.org/10.3892/mmr.2018.9150