Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays.

Authors :: Pownall ME
Saha MS
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1797, pp. 309-323.
Publication Year :: 2018
Abstract: Amphibian embryos have long served as an ideal model for teratogenicity testing. While whole-mount embryo observations can be utilized, histological observation of teratogenic phenotypes provides a wealth of additional information that can lead to mechanistic insights. In this chapter, detailed protocols for two methods of sectioning embryos as well as a guide for histological analysis is provided.

Subjects :: Animals
Biological Assay
Embryo, Nonmammalian drug effects
Paraffin Embedding
Xenopus laevis physiology
Embryo, Nonmammalian pathology
Embryonic Development
Histocytochemistry methods
Teratogens toxicity
Toxicity Tests methods
Xenopus laevis embryology

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