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Phenotypic and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Clones Carrying the Panton-Valentine Leukocidin Genes Disseminating in Iranian Hospitals.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2018 Dec; Vol. 24 (10), pp. 1543-1551. Date of Electronic Publication: 2018 Jun 12. - Publication Year :
- 2018
-
Abstract
- The spread of Panton-Valentine leukocidin (PVL)-carrying Staphylococcus aureus strains in both hospital and the community is a significant worldwide problem. The aim of the study was to investigate the clonal dissemination pattern of PVL-producing S. aureus strains isolated from hospitalized patients in Tehran, Iran. In this cross-sectional study, 70 PVL-carrying S. aureus strains were recovered from 240 clinical specimens and characterized by antibiotic susceptibility testing, agr typing, SCC mec typing, spa typing, multilocus sequence typing, and virulence and adhesion gene profiling. All the PVL-carrying S. aureus strains were confirmed as methicillin-resistant S. aureus (MRSA) and recovered from wounds (48.6%), blood (25.7%), exudate/pus (11.4%), sputum (8.6%), and body fluid (5.7%) samples. Among the 70 PVL-carrying S. aureus strains tested, 38 (54.3%) were positive for ant (4')-Ia gene, 27 (38.6%) for aac (6')-Ie/aph (2″) , 13 (18.6%) for msr (A), 13 (18.6%) for erm (C) , 13 (18.6%) for tet (M) , 11 (15.7%) for erm (A) , 10(14.3%) for msr (B), 9 (12.9%) for aph (3')-IIIa , 5 (7.1%) for mupA , and 2 (2.9%) for erm (B) genes. Five clonal complexes (CC) and nine different clones were detected in this study. The most frequent CC was CC22 (ST22) (42.8%) followed by CC30 (ST30) (21.5%), CC8 (ST8) (17.2%), CC1 (ST772) (11.4%), and CC80 (ST80) (7.1%). In this study, ST22-SCC mec IV/t852 was the predominant PVL-positive MRSA clone (20%), followed by ST8-SCC mec IV/t008 (17.2%), ST30-SCC mec IV/t019 (12.9%), ST22-SCC mec IV/t790 (11.4%), ST22-SCC mec IV/t005 (11.4%), ST30-SCC mec IV/t021 (8.6%), ST80-SCC mec IV/t044 (7.1%), ST772-SCC mec V/t657 (7.1%), and ST772-SCC mec V/t10795 (4.3%). Diversity in clonal types of PVL-carrying MRSA strains in our study supports the need to perform a systematic surveillance of PVL-positive MRSA strains.
- Subjects :
- Adolescent
Adult
Anti-Bacterial Agents pharmacology
Child
Child, Preschool
Cross-Sectional Studies
DNA, Bacterial genetics
Female
Humans
Iran
Male
Microbial Sensitivity Tests
Middle Aged
Multilocus Sequence Typing
Phenotype
Polymerase Chain Reaction
Young Adult
Bacterial Toxins genetics
Cross Infection microbiology
Exotoxins genetics
Leukocidins genetics
Methicillin-Resistant Staphylococcus aureus drug effects
Methicillin-Resistant Staphylococcus aureus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 24
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 29894277
- Full Text :
- https://doi.org/10.1089/mdr.2018.0033