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A WD40 Repeat Protein from Camellia sinensis Regulates Anthocyanin and Proanthocyanidin Accumulation through the Formation of MYB⁻bHLH⁻WD40 Ternary Complexes.
- Source :
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International journal of molecular sciences [Int J Mol Sci] 2018 Jun 06; Vol. 19 (6). Date of Electronic Publication: 2018 Jun 06. - Publication Year :
- 2018
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Abstract
- Flavan-3-ols and oligomeric proanthocyanidins (PAs) are the main nutritional polyphenols in green tea ( Camellia sinensis ), which provide numerous benefits to human health. To date, the regulatory mechanism of flavan-3-ol biosynthesis in green tea remains open to study. Herein, we report the characterization of a C. sinensis tryptophan-aspartic acid repeat protein (CsWD40) that interacts with myeloblastosis (MYB) and basic helix-loop-helix (bHLH) transcription factors (TFs) to regulate the biosynthesis of flavan-3-ols. Full length CsWD40 cDNA was cloned from leaves and was deduced to encode 342 amino acids. An in vitro yeast two-hybrid assay demonstrated that CsWD40 interacted with two bHLH TFs (CsGL3 and CsTT8) and two MYB TFs (CsAN2 and CsMYB5e). The overexpression of CsWD40 in Arabidopsis thaliana transparent testa glabra 1 ( ttg1 ) restored normal trichome and seed coat development. Ectopic expression of CsWD40 alone in tobacco resulted in a significant increase in the anthocyanins of transgenic petals. CsWD40 was then coexpressed with CsMYB5e in tobacco plants to increase levels of both anthocyanins and PAs. Furthermore, gene expression analysis revealed that CsWD40 expression in tea plants could be induced by several abiotic stresses. Taken together, these data provide solid evidence that CsWD40 partners with bHLH and MYB TFs to form ternary WBM complexes to regulate anthocyanin, PA biosynthesis, and trichome development.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Anthocyanins analysis
Anthocyanins genetics
Biosynthetic Pathways
Camellia sinensis chemistry
Camellia sinensis genetics
Flavonoids analysis
Flavonoids genetics
Flavonoids metabolism
Gene Expression Regulation, Plant
Plant Proteins chemistry
Plant Proteins genetics
Proanthocyanidins analysis
Proanthocyanidins genetics
Transcription Factors genetics
WD40 Repeats
Anthocyanins metabolism
Camellia sinensis metabolism
Plant Proteins metabolism
Proanthocyanidins metabolism
Protein Interaction Maps
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29882778
- Full Text :
- https://doi.org/10.3390/ijms19061686