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Auxin-Mediated Sterility Induction System for Longevity and Mating Studies in Caenorhabditis elegans .
- Source :
-
G3 (Bethesda, Md.) [G3 (Bethesda)] 2018 Jul 31; Vol. 8 (8), pp. 2655-2662. Date of Electronic Publication: 2018 Jul 31. - Publication Year :
- 2018
-
Abstract
- The ability to control both the means and timing of sexual reproduction provides a powerful tool to understand not only fertilization but also life history trade-offs resulting from sexual reproduction. However, precisely controlling fertilization has proved a major challenge across model systems. An ideal sterility induction system should be external, non-toxic, and reversible. Using the auxin-inducible degradation system targeting the spe-44 gene within the nematode Caenorhabditis elegans , we designed a means of externally inducing spermatogenesis arrest. We show that exposure to auxin during larval development induces both hermaphrodite self-sterility and male sterility. Moreover, male sterility can be reversed upon cessation of auxin exposure. The sterility induction system developed here has multiple applications in the fields of spermatogenesis and mating systems evolution. Importantly, this system is also a highly applicable tool for aging studies. In particular, we show that auxin-induced self-sterility is comparable to the commonly used chemically-induced FUdR sterility, while offering multiple benefits, including being less labor intensive, being non-toxic, and avoiding compound interactions with other experimental treatments.<br /> (Copyright © 2018 Kasimatis et al.)
- Subjects :
- Animals
Caenorhabditis elegans drug effects
Caenorhabditis elegans growth & development
Caenorhabditis elegans physiology
Caenorhabditis elegans Proteins genetics
Female
Infertility, Male chemically induced
Longevity
Male
Reproduction
Spermatogenesis genetics
Caenorhabditis elegans genetics
Indoleacetic Acids pharmacology
Infertility, Male genetics
Spermatogenesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2160-1836
- Volume :
- 8
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- G3 (Bethesda, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 29880556
- Full Text :
- https://doi.org/10.1534/g3.118.200278