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Altered Maturation of Medullary TEC in EphB-Deficient Thymi Is Recovered by RANK Signaling Stimulation.
- Source :
-
Frontiers in immunology [Front Immunol] 2018 May 09; Vol. 9, pp. 1020. Date of Electronic Publication: 2018 May 09 (Print Publication: 2018). - Publication Year :
- 2018
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Abstract
- In the present study, the relevance of EphB2 and EphB3 tyrosine kinase receptors for the maturation of medullary thymic epithelial cells (TECs) is analyzed. The absence of both molecules, but particularly that of EphB2, courses with altered maturation of medullary Cld3,4 <superscript>hi</superscript> SSEA1 <superscript>+</superscript> epithelial progenitor cells, mature medulla epithelial cells, defined by the expression of specific cell markers, including UEA1, MHCII, CD40, CD80, and AIRE, and reduced expansion of medullary islets. In vivo assays demonstrate that these changes are a consequence of the absence of EphBs in both TECs and thymocytes. On the other hand, the changes, that remains in the adult thymus, correlated well with reduced proportions of E15.5 Vγ5 <superscript>+</superscript> RANKL <superscript>+</superscript> cells in EphB-deficient thymi that could result in decreased stimulation of RANK <superscript>+</superscript> medullary TECs to mature, a fact that was confirmed by recovering of proportions of both CD40 <superscript>hi</superscript> CD80 <superscript>+</superscript> and MHCII <superscript>hi</superscript> UEA1 <superscript>+</superscript> mature medullary TECs of mutant E14.5 alymphoid thymic lobes by agonist anti-RANK antibody treatment. Accordingly, the effects of EphB deficiency on medullary TECs maturation are recovered by RANK stimulation.
- Subjects :
- Adrenal Medulla immunology
Animals
Cell Differentiation
Female
Mice
Receptor, EphB2 immunology
Receptor, EphB3 immunology
Thymocytes immunology
Adrenal Medulla cytology
Epithelial Cells immunology
Receptor Activator of Nuclear Factor-kappa B metabolism
Receptor, EphB2 genetics
Receptor, EphB3 genetics
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29867988
- Full Text :
- https://doi.org/10.3389/fimmu.2018.01020