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Dynamic structural states of ClpB involved in its disaggregation function.
- Source :
-
Nature communications [Nat Commun] 2018 Jun 01; Vol. 9 (1), pp. 2147. Date of Electronic Publication: 2018 Jun 01. - Publication Year :
- 2018
-
Abstract
- The ATP-dependent bacterial protein disaggregation machine, ClpB belonging to the AAA+ superfamily, refolds toxic protein aggregates into the native state in cooperation with the cognate Hsp70 partner. The ring-shaped hexamers of ClpB unfold and thread its protein substrate through the central pore. However, their function-related structural dynamics has remained elusive. Here we directly visualize ClpB using high-speed atomic force microscopy (HS-AFM) to gain a mechanistic insight into its disaggregation function. The HS-AFM movies demonstrate massive conformational changes of the hexameric ring during ATP hydrolysis, from a round ring to a spiral and even to a pair of twisted half-spirals. HS-AFM observations of Walker-motif mutants unveil crucial roles of ATP binding and hydrolysis in the oligomer formation and structural dynamics. Furthermore, repressed and hyperactive mutations result in significantly different oligomeric forms. These results provide a comprehensive view for the ATP-driven oligomeric-state transitions that enable ClpB to disentangle protein aggregates.
- Subjects :
- Adenosine Triphosphate metabolism
Bacterial Proteins chemistry
Bacterial Proteins genetics
Endopeptidase Clp chemistry
Endopeptidase Clp genetics
Heat-Shock Proteins chemistry
Heat-Shock Proteins genetics
Microscopy, Atomic Force
Mutation
Protein Aggregates
Protein Aggregation, Pathological
Protein Binding
Protein Conformation
Protein Multimerization
Thermus thermophilus genetics
Bacterial Proteins metabolism
Endopeptidase Clp metabolism
Heat-Shock Proteins metabolism
Thermus thermophilus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 29858573
- Full Text :
- https://doi.org/10.1038/s41467-018-04587-w