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Enhanced survival following oral and systemic Salmonella enterica serovar Typhimurium infection in polymeric immunoglobulin receptor knockout mice.
- Source :
-
PloS one [PLoS One] 2018 Jun 01; Vol. 13 (6), pp. e0198434. Date of Electronic Publication: 2018 Jun 01 (Print Publication: 2018). - Publication Year :
- 2018
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Abstract
- Background: Polymeric immunoglobulin receptor (pIgR) transport of secretory immunoglobulin A (SIgA) to mucosal surfaces is thought to promote gut integrity and immunity to Salmonella enterica serovar Typhimurium (S. Typhimurium), an invasive pathogen in mice. To elucidate potential mechanisms, we assessed intestinal barrier function and both oral and systemic S. Typhimurium virulence in pIgR knockout (KO) and wildtype (WT) mice.<br />Methods: In uninfected animals, we harvested jejunal segments for Ussing chamber analyses of transepithelial resistance (TER); mesenteric lymph nodes (mLN) for bacterial culture; and serum and stool for IgA. Separately, we infected mice either orally or intravenously (IV) with S. Typhimurium to compare colonization, tissue dynamics, and inflammation between KOs and WTs.<br />Results: Uninfected KOs displayed decreased TER and dramatically increased serum IgA and decreased fecal IgA vs. WT; however, KO mLNs yielded fewer bacterial counts. Remarkably, WTs challenged orally with S. Typhimurium exhibited increased splenomegaly, tissue colonization, and pro-inflammatory cytokines vs. pIgR KOs, which showed increased survival following either oral or IV infection.<br />Conclusions: Absence of pIgR compromises gut integrity but does not exacerbate bacterial translocation nor S. Typhimurium infection. These findings raise the possibility that immune adaptation to increased gut permeability and elevated serum IgA in the setting of SIgA deficiency provides compensatory protection against invasive gut pathogens.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Administration, Oral
Animals
Cytokines blood
Feces chemistry
Immunoglobulin A analysis
Immunoglobulin A blood
Injections, Intravenous
Intestines pathology
Lymph Nodes microbiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Polymeric Immunoglobulin deficiency
Salmonella Infections, Animal microbiology
Salmonella Infections, Animal mortality
Salmonella enterica physiology
Splenomegaly etiology
Survival Rate
Receptors, Polymeric Immunoglobulin genetics
Salmonella Infections, Animal pathology
Salmonella enterica pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 13
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 29856838
- Full Text :
- https://doi.org/10.1371/journal.pone.0198434