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The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse.
- Source :
-
Scientific reports [Sci Rep] 2018 May 30; Vol. 8 (1), pp. 8417. Date of Electronic Publication: 2018 May 30. - Publication Year :
- 2018
-
Abstract
- The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two long (LAP*, LAP) and one truncated (LIP), with the same C-terminal bZip dimerization domain. The long C/EBPβ isoforms are considered gene activators, whereas the LIP isoform reportedly acts as a dominant-negative repressor. Here, we tested the putative repressor functions of the C/EBPβ LIP isoform in mice by comparing monoallelic WT or LIP knockin mice with Cebpb knockout mice, in combination with monoallelic Cebpa mice. The C/EBPβ LIP isoform was sufficient to function in coordination with C/EBPα in murine development, adipose tissue and sebocyte differentiation, and female fertility. Thus, the C/EBPβ LIP isoform likely has more physiological functions than its currently known role as a dominant-negative inhibitor, which are more complex than anticipated.
- Subjects :
- 3T3-L1 Cells
Adipose Tissue metabolism
Alleles
Animals
CCAAT-Enhancer-Binding Protein-beta deficiency
Female
Fertility genetics
Gene Knockout Techniques
Homeostasis
Mice
Phenotype
Protein Isoforms deficiency
Protein Isoforms genetics
Protein Isoforms metabolism
Skin metabolism
CCAAT-Enhancer-Binding Protein-beta genetics
CCAAT-Enhancer-Binding Protein-beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29849099
- Full Text :
- https://doi.org/10.1038/s41598-018-26579-y