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A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2018 Jul 26; Vol. 56 (8). Date of Electronic Publication: 2018 Jul 26 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis requires bacterial culture, which can be time-consuming. A reliable rapid serological tool is greatly needed for disease surveillance and diagnosis. We previously demonstrated by enzyme-linked immunosorbent assay (ELISA) that a hemolysin-coregulated protein (Hcp1) is a promising target for serodiagnosis of melioidosis. In this study, we developed a rapid immunochromatography test (ICT) using Hcp1 as the target antigen (Hcp1-ICT). We evaluated this test for specific antibody detection using serum samples obtained from 4 groups of human subjects, including the following: (i) 487 culture-confirmed melioidosis patients from four hospitals in northeast Thailand; (ii) 202 healthy donors from northeast Thailand; (iii) 90 U.S. healthy donors; and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors were 86.1% and 100%, respectively, and the specificity for other infections was 91.8%. The results of the Hcp1-ICT demonstrated 92.4% agreement with the Hcp1-ELISA results with a kappa value of 0.829, indicating that the method is much improved compared with the current serological method, the indirect hemagglutination assay (IHA) (69.5% sensitivity and 67.6% specificity for Thais). The Hcp1-ICT represents a potential point-of-care (POC) test and may be used to replace the IHA for screening of melioidosis in hospitals as well as in resource-limited areas.<br /> (Copyright © 2018 Phokrai et al.)
- Subjects :
- Antibodies, Bacterial blood
Burkholderia pseudomallei immunology
Hemagglutination Tests
Humans
Point-of-Care Testing
Sensitivity and Specificity
Thailand
Bacterial Proteins immunology
Burkholderia pseudomallei isolation & purification
Chromatography, Affinity
Enzyme-Linked Immunosorbent Assay
Melioidosis diagnosis
Serologic Tests methods
Virulence Factors immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 56
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29848565
- Full Text :
- https://doi.org/10.1128/JCM.00346-18