Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.

Authors :: Kimura M
Isobe S
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1795, pp. 93-99.
Publication Year :: 2018
Abstract: Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency of Agrobacterium-mediated transformation of a model legume, Lotus japonicus. Moreover, we explain the transformation protocol using chloroxynil, a phenolic compound that improves the efficiency.

Subjects :: Fabaceae drug effects
Gene Expression
Gene Transfer Techniques
Genes, Reporter
Seeds
Agrobacterium tumefaciens physiology
Drug Discovery methods
Fabaceae genetics
Small Molecule Libraries
Transformation, Genetic drug effects

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