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Optimization of the in vitro fertilization protocol for frozen epididymal sperm with low fertilization ability in Ban-A native Vietnamese pigs.
- Source :
-
Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2018 Aug; Vol. 89 (8), pp. 1079-1084. Date of Electronic Publication: 2018 May 29. - Publication Year :
- 2018
-
Abstract
- The aim of the present study was to improve the penetration during in vitro fertilization (IVF) of a frozen lot of epididymal sperm with a notoriously low fertilization ability of a Ban boar which is a native Vietnamese breed by optimizing different parameters of the IVF system. In Experiment 1, we determined that Pig-fertilization medium was superior medium to Tyrode's albumin lactate pyruvate-polyvinyl alcohol medium for IVF and defined the optimum the sperm concentration (1 × 10 <superscript>6</superscript>  sperm/ml). In Experiment 2, we clarified that partial removal of cumulus cells from cumulus-oocyte complexes by hyaluronidase treatment before IVF enhances sperm penetration, whereas complete cumulus removal reduces penetration. Finally, in Experiment 3 the elevation of concentration of caffeine in Pig-fertilization medium from 2 to 5 mmol/L and the prolongation of the co-culture of gametes from 3 to 5 hr significantly increased the total penetration rate from 15.2% to over 50%. In conclusion, the combination of partial oocyte denudation, an elevated caffeine concentration in Pig-fertilization medium and an extended interval of IVF with using an optimized sperm concentration was a potent way to improve the fertilization results for a frozen epididymal Ban sperm lot with low fertility.<br /> (© 2018 Japanese Society of Animal Science.)
Details
- Language :
- English
- ISSN :
- 1740-0929
- Volume :
- 89
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Animal science journal = Nihon chikusan Gakkaiho
- Publication Type :
- Academic Journal
- Accession number :
- 29808519
- Full Text :
- https://doi.org/10.1111/asj.13045