Back to Search Start Over

Optimization of the in vitro fertilization protocol for frozen epididymal sperm with low fertilization ability in Ban-A native Vietnamese pigs.

Authors :
Linh NV
Somfai T
Nguyen TH
Nhung NT
Hong NT
Dat NT
Thinh NH
Van NK
Quyen DV
Chu HH
Son NT
Kikuchi K
Source :
Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2018 Aug; Vol. 89 (8), pp. 1079-1084. Date of Electronic Publication: 2018 May 29.
Publication Year :
2018

Abstract

The aim of the present study was to improve the penetration during in vitro fertilization (IVF) of a frozen lot of epididymal sperm with a notoriously low fertilization ability of a Ban boar which is a native Vietnamese breed by optimizing different parameters of the IVF system. In Experiment 1, we determined that Pig-fertilization medium was superior medium to Tyrode's albumin lactate pyruvate-polyvinyl alcohol medium for IVF and defined the optimum the sperm concentration (1 × 10 <superscript>6</superscript>  sperm/ml). In Experiment 2, we clarified that partial removal of cumulus cells from cumulus-oocyte complexes by hyaluronidase treatment before IVF enhances sperm penetration, whereas complete cumulus removal reduces penetration. Finally, in Experiment 3 the elevation of concentration of caffeine in Pig-fertilization medium from 2 to 5 mmol/L and the prolongation of the co-culture of gametes from 3 to 5 hr significantly increased the total penetration rate from 15.2% to over 50%. In conclusion, the combination of partial oocyte denudation, an elevated caffeine concentration in Pig-fertilization medium and an extended interval of IVF with using an optimized sperm concentration was a potent way to improve the fertilization results for a frozen epididymal Ban sperm lot with low fertility.<br /> (© 2018 Japanese Society of Animal Science.)

Details

Language :
English
ISSN :
1740-0929
Volume :
89
Issue :
8
Database :
MEDLINE
Journal :
Animal science journal = Nihon chikusan Gakkaiho
Publication Type :
Academic Journal
Accession number :
29808519
Full Text :
https://doi.org/10.1111/asj.13045